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Peptide and Protein Engineering Laboratory

420 W. 12^th Ave. Suite 306

Columbus, OH 43210

Contact:

Dr. Pravin Kaumaya                       or                                Jay Overholser

Director                                                                                Manager

Tel:  614-292-7028                                                             614-292-0736          

Fax: 614-688-8586 

Email: kaumaya.1@osu.edu                                              overholser.18@osu.edu

The Peptide and Protein Engineering Laboratory is committed to providing affordable peptides to support the rapidly expanding areas of immunology, biochemistry, molecular biology, and biotechnology for Medical Center members, The Ohio State University researchers, and will consider external academic contracts during periods of reduced demand.

The Laboratory’s mission includes

• Low cost synthesis, purification, and characterization of peptides
• Expertise in selection, design, and applications
• Generate awareness and enthusiasm for peptide synthesis methodology
• Encourage peptide research
• State-of-the-art peptide laboratory

The Laboratory can provide synthetic peptides for:

• Immunogenic studies
• Protein and peptide engineering studies
• Receptor studies
• Structure-activity determination studies
• Enzyme substrates
• Diagnostic reagents
• Molecular and cell biology
• Biochemical and Chemical studies

The Laboratory can provide expertise with:

• Prediction and selection of potential antigenic peptides
• Computerized sequence prediction by variety of methods is available upon request.  This service will allow investigators to target peptide sequence for analysis.
• Complete project of sequence design, peptide synthesis, immunization and antibody production in mice and rabbits.
• Peptide-carrier conjugation
• Cyclization using disulfide bonds.

Custom peptides:

1. Standard Reagent Grade:  A standard reagent grade undergoes no purification after deprotection and cleavage.  Minimum purity: 60% on HPLC.  Quantity: 100-200 mg.
2. Immunological Reagent Grade: Purified >80% on HPLC.  Quantity: 100-100 mg.
3. Bioassay Reagents Peptide for Biological Studies: Extensive purification >95% on HPLC and MS.  Quantity: 20-50 mg.
4. Multiple Overlapping Peptide:  A multiple overlapping peptide synthesizer is available, which allows large number of infinitely different peptides; up to 96 different peptides can be synthesized in one run.  Overlapping sequences of an entire protein can now be obtained in quantities varying between 10-20 mg.    

Peptide Synthesis

Peptides are complex molecules and each peptide sequence is unique with regard to its chemical and physical properties. Peptides are synthesized by the solid-phase method using Fmoc chemistry.  We have two Biosearch 9600 synthesizers capable of producing over 200 my crude peptide in single synthesis, and one Advanced Chemtech MPS 396 multiple peptide synthesizer capable of synthesizing 40 different peptides in single synthesis.  We have state of the art purification instrumentation backed by BIO-RAD size exclusion and three Waters HPLC instruments. The peptide synthesis service provides a complete spectrum of services that include, peptide design, epitope prediction, synthesis, cleavage, HPLC purification, peptide modifications, cyclization and mass analysis for quality assurance. Before submitting your peptide sequences, it is advisable to consider the following important aspects: Every peptide sequence is a unique one. Some peptides sequences are difficult to synthesize although, we attempt to synthesize every peptide sequence.

• Peptides with multiple cysteines, methionine, arginine and tryptophan residues can often be difficult to synthesize.
• Avoid a proline residue at the C-terminal of the peptide.

Peptide purification and analysis

Every peptide we synthesize is analyzed on reversed phase HPLC  in our laboratory and characterized by either MALDI or electro-spray mass spectrometry in Campus Instrumentation Center (CCIC) of The Ohio State University

Modification of Synthetic Peptides

Our laboratory routinely synthesizes peptides with the following modifications: peptides containing unusual or modified amino acids (e.g., phospho-Tyr, phospho-Ser/Thr, D-amino acids or other analogs), biotinylation at N- or C- terminals,  FITIC addition, N-acetylation, peptides amides, and peptide cyclization via disulfide bonds.

Peptide Conjugation to a Carrier Protein for Immunization

If you have protein of interest, then we can perform epitope prediction using computer programming coupled with our knowledge of the peptide design and can predict best epitope for the generation of antibodies.   Over the years in the field, we have developed universal T_H epitope, which can be added to the B-cell epitope for enhanced immune response.  The exact cost of the peptide design and synthesis will depend on the sequence of amino acids required, as different amino acids vary greatly (10-fold) in price. The incorporation of an N-terminal modification (e.g. acetylation, biotinylation or FITIC) or cyclization using disulfide bonding. 

 Epitope Design and Antibody production:

Over the years we have developed the unique T_H epitope in our laboratory to present the B-cell epitope for the antibody production.  We can use our expertise to design the epitope, synthesize, immunize rabbits/mice, generate and purify antibodies for research purpose.  Please setup the meeting with Dr. Kaumaya for such a project.  We have successfully completed several antibody production projects for researchers at OSU.

Please contact for the confidential quote.  We would like to know your amino acid sequence, desired purity and quantity.